Principle of Flow Cytometry

The basic principle of flow cytometry is the passage of cells in single file in front of a laser so they can be detected, counted and sorted. Scattering of light and emission of fluorescence occur when a laser beam hits the cells moving in a directed fluid stream. Light scattering by these cells and cellular components occurs due to their structural and morphological properties.

Brief Introduction

MoniSight scientific flow cytometer：The performance is comparable to the highest standard of imported flow cytometer, dual laser four/six/eight color options, standard 40 tube/96-well plate high throughput injector, automatic injection, compact, comprehensive, automatic cleaning and maintenance, simple operation, with automatic analysis function, it is setting a new standard for laboratory-accessible flow cytometry.

MoniSight (Y/B) scientific flow cytometer: It is equipped with a unique 561nm all-solid-state laser, perfect for PE family dye/mCherry detection. Excitable fluoresceine include: PE, PE-Texas Red, PE-Cy5, PE-Cy7, RFP, mCherry, etc., greatly improve the detection sensitivity and resolution of PE, PE family dyes and mCherry and other red fluorescent proteins. The 561nm laser can also identify the transfection effect of RFP and track the transfected cells. The bimolecular fluorescence complementary system (BiFC) based on mCherry can detect the interaction between proteins in cells, which is simple, rapid, intuitive, sensitive and localization.

Product Characteristics

1、 Flexible configuration

1.1  MoniSight scientific flow cytometer：

Dual laser（488nm、638nm）four/six/eight colors;

1.2  MoniSight (Y/B) scientific flow cytometer：

Single laser（561nm）five colors, or dual laser（561nm、488nm）six colors/eight colors.

2、High performance 

2.1 Fast detection speed: 50,000 events/s. 

2.2 High sensitivity: Fluorescence sensitivity of eight-peak rainbow microspheres:

• FITC≤30 MESF

• PE≤20 MESF

• APC≤20 MESF

2.3 High resolution: Instrument main channel resolution: CV≤2%

3、Automatic injection

Automatic sampling: support 96-well plates, 40 12×75mm flow tubes, and a variety of tubes (such as 1.5mL, 2mL EP tubes, etc.), automatic cleaning between samples.

4、Intelligent and simple 

Intelligent mixing and cleaning, fixed optical path without correction.

Simple operation, save time: 10min fast startup, automatic shutdown, 2min to achieve collection, analysis and complete process report, simple process, save time and save sheath fluid.

5、Precise absolute counting 

Compatible with volume method and microsphere method absolute counting.

Built-in high-precision pump, precise quantification of sample flow volume, direct calculation of absolute counting, counting accuracy ≤±5%, no need to count absolute microspheres.

Application Fields

Broad application fields，such as:

1、Cell cycle analysis of biological samples

2、Annexin V/PI analysis of cell apoptosis

3、Cell apoptosis (mitochondrial membrane potential) analysis

4、Lymph subgroup detection

5、Cell factors analysis

6、Sperm fragments detection

1、Cell cycle analysis of biological samples

2、Annexin V/PI analysis of cell apoptosis

3、Cell apoptosis (mitochondrial membrane potential) analysis

4、Lymph subgroup detection

5、Cell factors analysis

6、Sperm fragments detection