Brief Introduction

The SA3800 spectral analyzer improves sensitivity and simplifies application design, workflow, and analysis over conventional flow cytometers. This is achieved using spectral analysis technology, automation throughout the system, advanced electronics, and patented optical technologies, unique to Sony systems.

Spectral Analysis Technology

Spectral analysis technology is the foundation of the SA3800 system. Spectral flow cytometry streamlines workflow and yields better data by collecting the entire visual spectrum of light. In conventional systems, overlapping fluorescence is subtracted using color compensation, so less light is collected. Instead, spectral flow cytometers sum the fluorescence together and then use unmixing to mathematically separate the colors. This powerful capability also simplifies workflow, including panel design, and improves visualization of autofluorescence.

A unique prism collection system delivers emitted light to a 32-channel PMT. This produces 34 data points of signal detection for fluorescence and bright autofluorescence to achieve accurate visualizations of fluorescent populations. Researchers can see the complete spectral fingerprint of each fluorochrome from 420 nm to 800 nm.

Novel 3D AutoSampler Technology

The novel 3D AutoSampler uses a fixed probe and moves the plate in horizontal and vertical directions to minimize sample-to-sample cross-contamination and speed up cleaning.

Sensors on the probe enable the system to accurately move the sample to the probe, calculate height of the tube or plate, and automatically recoil the probe if it touches the container or base plate surface. This unique design is aimed at reducing cross-contamination and clogs, and allows for automatic recovery when flow problems are encountered. It also enables faster cleaning between samples.

Product Characteristics

• Uses spectral analysis technology to optimize sensitivity while simplifying application design and workflow.

• Unique 3D plate motion technology enables the plate (and sample) to move in horizontal and vertical directions to eliminate the need for cumbersome fluidics and their associated sample-to-sample cross-contamination. In addition, built-in, adjustable mixing mechanisms help maintain sample heterogeneity and optimal CVs between samples.

• The SA3800 features automation across the workflow, including automated alignment and a software calibration wizard to simplify operation and improve the reliability of results.

• Spectral technology in the SA3800 optimizes sensitivity and enhances dim signal detection by collecting photons at wavelengths from 420 nm to 800 nm. It also simplifies multicolor application design, workflow, and analysis for experienced and novice users.
  

Phenotyping of B-Cells, Effector T-Cell, Helper T-Cells, and Monocytes Using a Single Blue Laser

Spectrum Detection of Various Fluorochromes on Each of the Four Lasers

8-color TBNK Panel on Human Peripheral Blood Sample